The fluorescent signal only is produced after the proteins have interacted, which is generally in the order of hours.
This produces an increase in the fluorescent signal dependent on glucose concentration.
The spots in the right image are much less clear against the high background, but the fluorescent signals are still measurable.
Together the probe and primers allow for a specific fluorescent signal to be emitted during amplification.
Once the reaction mix is washed away, the fluorescent signal will identify the base at position one.
When the excess fragments are washed away, a fluorescent signal can be read.
Numerous antibodies may bind to microtubules in order to amplify the fluorescent signal.
A chemical is added to be converted by the enzyme into a color or fluorescent or electrochemical signal.
A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal.
Any reaction between the probe and the immobilised protein emits a fluorescent signal that is read by a laser scanner.