Diabetic ulcer fibroblasts are usually large and widely spread in the culture flask compared to the spindle shaped morphology of the fibroblasts in age-matched controls.

Adherent cells, for example many mammalian cell lines, grow attached to a surface such as the bottom of the culture flask.

The cell population was then expanded by sequentially subculturing into 25-cm 2tissue culture flasks, then to 75-cm 2tissue culture flasks.

This is the realm of pure abstraction: no test tubes here, no culture flasks or Petri dishes, and no visible hint of the likes of me.

Once a week, cells were re-plated at 20% density into 75-cm 2tissue culture flasks.

Treatment with pertussis toxin was performed on attached cells in culture flasks for two hours before detachment.

He started a culture flask, made a control slide and examined it under the microscope.

Following a further five minute incubation, the contents of the cuvette were transferred directly to a culture flask containing 4 mls of SDM-79 or Cunningham's medium.

For cytotoxicity studies, 25 cm 2tissue culture flasks were seeded with 0.66 million NG108 cells in 10 ml culture medium 24 h prior to the experiment.

If A549 cells are cultured in vitro, they grow as monolayer cells, adherent or attaching to the culture flask.