The levels were nevertheless lower than those detected in undifferentiated cell extracts.
The antigens are either from cell extracts or recombinant.
The second type of experiment consisted of in vitro experiments done on cell extracts.
Because the enzyme has only been assayed in a crude cell extract, the exact location of it in the plant has not been determined.
Eukaryotic cell extracts may also be used in other cell-free systems.
Nuclear cell extracts (3 μg) were added to the wells and assayed for either Rel A or p50 binding as per the manufacturer's instructions.
The above experiments were performed with total cell extracts made in the presence of 0.5 M KCl.
The remainder of the cell extract was used for immunoprecipitation.
After 48-72 hours, cell extracts were prepared and assayed for CAT activity (36).
Cytoplasmic cell extract (S100) was prepared as described previously (7).
