Prolyl isomerase activity was first discovered using a chymotrypsin-based assay.
These double stranded mutants are then screened for the desired optimized function using a relevant assay.
To search for such a protein, Dr. Ross and his colleagues used a special assay that screens for proteins that interact.
Termites were later collected from the field and tested for the rabbit-IgG markers using a rabbit-IgG-specific assay.
Therefore, we used a GST-pulldown assay to determine if the proteins could interact in vitro .
Orthogonal testing: Confirmed hits are assayed using a different assay which is usually closer to the target physiological condition or using a different technology.
STS enzyme deficiency is confirmed using a clinically available biochemical assay.
After allowing time for protein synthesis, β-galactosidase activity is assayed using a simple colorimetric assay.
Fibrinogen was measured using a quantitative assay of clotting time compared to a known standard.
