For atypical splice junctions, a higher level of supporting data was required (see below).

Even for transcript structures based upon EST data, the number of atypical splice junctions is probably an underestimate.

Splicing enhancers are sites to which splicing activator proteins bind, increasing the probability that a nearby site will be used as a splice junction.

Two alternative transcripts for this gene are supported by cDNA data, and both appear to contain an unconventional, ambiguous splice junction.

The dynamic programming alone is accurate in determining splice junctions but computationally expensive.

Each node comprises the set of spans that share common splice junctions.

In each case the splices result in appropriate splice junctions and a single open reading frame.

Furthermore, recognizing that the responsible mutation may be present in one of the intron-exon splice junctions, it is also necessary to obtain the corresponding genomic sequence.

Most of the splice junctions follow the GT/AG rule.

EJCs are multiprotein complexes that assemble during splicing at a position about 20-24 nucleotides upstream from the splice junction.