In the R2 protein the iron centre is located about 10Å from the protein surface.

When deciding the linker fusion site on the protein surface, there are three main considerations.

However, they may also bind to the rest of the protein surface.

Water establishes a concentration gradient around the protein, with the highest concentration at the protein surface.

The hydrophobic patches on the protein surface generate highly ordered water shells.

The iron of the active site is located at the bottom of a narrow channel, approximately 15 Å from the protein surface.

JPD offers information about electrostatic potential and curvature on protein surface.

This tool helps in identifying the geometric properties of protein pockets which are assumable position on protein surface.

If enough of the protein surface is hydrophilic, the protein can be dissolved in water.

They become functional when folding brings them together on the protein surface.