From 20 to 200 g of total RNA was used.
Total RNA was then extracted according to the manufacturer's instructions.
Total RNA was heated in a 65 C water bath for 10 minutes.
Ten micrograms of total RNA were used for northern blot analysis.
Our first goal was to determine a reasonable lower bound for total RNA that could successfully be used in the standard protocol.
Thus, 125 ng of total RNA can be used for one slide hybridization.
Total RNA is purified from the specimen of interest.
Reverse transcription was performed with 1-2 μg of total RNA.
One protocol for analyzing gene expression involves first isolating total RNA.
The typical yield of total RNA was 50-80 μg/100 mg leaf tissue.
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