Lysates were precleared with normal rabbit serum and equal amounts incubated at 37 C for the times indicated.

After preclearing again with normal rabbit serum, class I molecules were immunoprecipitated with monoclonal antibody W6/32.

Negative controls were established by replacing the primary antibody with PBS and normal mouse or rabbit serum.

The rabbit serum was applied to an affinity column to purify the anti-III 1-C antibodies.

In the "control" test tube we insert only the detector system and the fresh rabbit serum.

In the "control" test tube we ever observe hemolisis (if the rabbit serum and the detector system are effective).

Normal rabbit serum was used at 1:200 as a negative control.

Preimmune rabbit serum was used in place of SPAM1 antibody as a control.

Rats injected with purified PON1 from rabbit serum were more resistant to acute cholinergic activity than the control rats.

Immunoprecipitation with 1 ug of normal rabbit serum served as a negative control.