Because primer extension is based on the highly accurate DNA polymerase enzyme, the method is generally very reliable.

The flexibility and specificity of primer extension make it amenable to high throughput analysis.

The longest primer extension performed by a ribozyme polymerase was 20 bases.

Single nucleotide polymorphism determination using primer extension and time-of-flight mass spectrometry.

In the end, we will still need to conduct experiments such as primer extension to distinguish real from false-positive predictions.

An oligonucleotide containing the desired mutation is used for primer extension.

Unlike other methods, cassette mutagenesis need not involve primer extension using DNA polymerase.

RNAs were precipitated and analyzed by primer extension as indicated below.

After 10 minutes, the reactions were stopped and the transcripts produced analyzed by primer extension, polyacrylamide-urea gel electrophoresis and autoradiography.

Due to the application of microfluidics, neither initial biotin nor reagent treatment is necessary prior to primer extension.