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A complementary RNA copy of DNA is made.
Transcription is the process where genetic information in DNA is used to produce a complementary RNA strand.
From there, the RNA is able to act as a template for complementary RNA synthesis, immediately.
The secondary structure is the result of base-pairing of complementary RNA nucleotides, and causes a hairpin-like structure.
The positive- or plus-sense genome, then makes viral complementary RNA (vcRNA) copies of itself.
The goal for the design was to find derivatives that possess high binding affinities with complementary RNA and/or DNA strands.
Biotinylated complementary RNA was prepared from 10 μg of cDNA and then fragmented to approximately 50 to 100 nucleotides.
T7 RNA polymerase continuously produces complementary RNA strands of this template which results in amplification.
As noted above, the probe is either a labeled complementary DNA or, now most commonly, a complementary RNA (riboprobe).
During the following so-called elongation phase, this nucleotidyl transfer reaction is repeated with subsequent NTPs to generate the complementary RNA product.
It is a type I system because the toxin is neutralised by a complementary RNA, rather than a partnered protein (type II toxin-antitoxin).
These domains are also found on riboswitches, but unlike riboswitches, the sensor domain only binds complementary RNA or DNA strands as opposed to small molecules.
There is no predicted stem loop structure on QRICH2 mRNA because there isn't any complementary RNA sequences.
On the lagging strand template, a primase "reads" the template DNA and initiates synthesis of a short complementary RNA primer.
Replicate hybridizations in the datasets included independently generated complementary RNA (cRNA) preparations from the same starting total RNA.
The complementary RNA is created in the opposite direction, in the 5' 3' direction, matching the sequence of the sense strand with the exception of switching uracil for thymine.
Lechner used a self pairing stem-loop which inhibited translation of a prokaryotic RNA, unless a complementary RNA sequence (anti-inhibitor) was present.
A particular gene is 'transcribed' into mRNA; that is, a complementary RNA strand is synthesized by base-pairing with one of the two DNA strands.
Hybridisation of sections took place in a mixture containing 1x10 counts per min per section of S-labelled ET-1 complementary RNA probe for 14 h at 42 C.
The enzyme then progresses along the template strand in the 3' to 5' direction, synthesizing a complementary RNA molecule with elongation occurring in the 5' to 3' direction.
Immunohistochemistry and in-situ hybridisation were done with polyclonal antisera to ET-1 and its precursor big ET-1, and complementary RNA probes for preproET-1.
This bridge forms a bi-cyclic structure that locks the ribose conformation, and is integral to the high stability and affinity of the LNA to its complementary RNA sequence.
Some authors have used the term micRNA (mRNA-interfering complementary RNA) to refer to these RNAs but it is not widely used.
Here, RNA-encoding genes control mRNA abundance or translation by producing a complementary RNA that anneals to an mRNA.
Some viruses (e.g., Coronaviridae) have positive-sense genomes that can act as mRNA and be used directly to synthesize proteins without the help of a complementary RNA intermediate.
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