Hybridization - refers to the process by which the single-stranded DNA or RNA preparation is added to the array surface, in solution, and potentially anneals to the complementary probe.

RNA preparations were run in duplicate and each sample was hybridized to triplicate arrays.

Because we created groups of samples diluted from the same individual RNA preparations, any group differences reflect differences in labeling and handling of the samples.

RNA preparation and northern blot analysis Fibroblasts were grown to confluence in 10% FBS/DMEM and then incubated in serum-free medium.

RNA preparation and northern blot analyses Total RNA from MCF-7 cells and human breast tumors was prepared as described [ 33 ] .

Furthermore, the level of resolution is relatively low when RNA preparations from whole animals are used.

In their study, the same RNA preparation was used as a template for distinct cDNA/cRNA amplifications and hybridizations.

The β-actin RT-PCR served as positive control and gave strong signals in all cases, indicating that both RNA preparation and cDNA synthesis were successful.

The calcium phosphate-DNA was left on the cells for 12-16 hours then the medium was changed and the cells harvested 48 hours after transfection for RNA preparation.

However, both messages appear to be present at the same level in the total RNA preparation (fifth lane).