Two-dimensional chromatography represents the most thorough and rigorous approach to evaluation of the proteome.

The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography.

Two-dimensional chromatography is a type of chromatographic technique in which the injected sample is separated by passing through two different separation stages.

Double-way paper chromatography, also called two-dimensional chromatography, involves using two solvents and rotating the paper 90 in between.

The spot capacity (analogous to peak capacity in HPLC) can be increased by developing the plate with two different solvents, using two-dimensional chromatography.