transfect

Importantly, we report for the first time the ability to directly and effectively transfect cells in a growth arrested state.

Primary cells, for example stem cells, especially fall into this category, although many other cell lines are also difficult to transfect.

Nucleofection is a method to transfer substrates into mammalian cells so far considered difficult or even impossible to transfect.

To do this, dilutions of the library are used to transfect cultures of E. coli of known concentrations.

Thus, nucleofection provides the ability to transfect even non-dividing cells, such as neuron and resting blood cells.

Therefore, transfection of naked DNA using electroporation might offer a possibility to transfect adenoviral-resistant tumours.

HEK 293 cells are very easy to grow and transfect very readily and have been widely used in cell biology research for many years.

Bacterial spheroplasts, with suitable recombinant DNA inserted into it, can be used to transfect animal cells.

Adenoviruses modified to transfect tumor cells with harmless yet distinct genes (such as luciferase) have proven capable of early detection.

Different scAAV and AAV serotypes can efficiently transfect a variety of cellular targets.

Because of the relatively high transfection rate observed with polybrene, we examined the relative efficiencies of various other polycations for their ability to transfect CHO cells.

Plasmid DNA alone is not very efficient transfectant, therefore the electroporation offers a possibility to transfect tumours resistant to viral infection [ 11 12 ] .

(iv) Regulation any time: Although we have not tested this specifically, one can, in principle, transfect the virus-infected cell with dsRNA at any time point during infection.

The cDNAs for the two rat transporter chains TAP1a and TAP2a were used individually or as a mixture to transfect T2 cells.

It could then be shown that His2 DNA could transfect a wide variety of haloarchaeal species even though the cells were not susceptible to infection by His2 virus.

Plasmid DNA was prepared by alkaline lysis, purified with Qiagen column (Diagen) and used to transfect COS-7 cells for eukaryotic expression.

However, they are extremely easy to work with, being straightforward to culture and to transfect, and so can be used in experiments in which the behavior of the cell itself is not of interest.

This is not to suggest lentiviral vectors do not efficiently transfect cells in vivo as well as rAAV vectors with transcripts less than 4 kb, though.

Even if these combined protocols seems to be slightly less toxic, there is still much improvement needed to compete with the new generations of adenoviral vectors as a method to transfect muscle in patients.

The lipofectamine reagent (Canadian Life Technologies) was used to transfect the expression plasmid into CHO, McArdle RH7777, and McNtcp.24 cells.

Magnetofection works for primary cells and hard to transfect cells that are not dividing or slowly dividing, meaning that the genetic materials can go to the cell nucleus without cell division.

In addition to being able to transfect cells with DNA plasmids coding for fluorescent proteins, the gene gun can be adapted to deliver a wide variety of vital dyes to cells.

Limitations of these competing reagents have been reported to include: the lack of ability to transfect some cell types, the lack of robust active targeting capabilities, incompatibility with animal models, and toxicity.

In Osteoarthritis gene therapy, ex vivo method makes it possible to transfect not only the cells of the synovial lining of joints but also articular chondrocytes and chondroprogenitor cells in cartilage.

Trimethylchitosan, or quaternised chitosan, has been shown to transfect breast cancer cells, with increased degree of trimethylation increasing the cytotoxicity; at approximately 50% trimethylation, the derivative is the most efficient at gene delivery.