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The most common method to isolate individual cells and produce a pure culture is to prepare a streak plate.
A streak plate can refer to:
It comes in shades of blue and green and, when rubbed on a streak plate, is pale green to white in color.
Streak testing is constrained by the hardness of the mineral, as those harder than 7 powder the streak plate instead.
The most common way of testing this property is done with a streak plate, which is made out of porcelain and coloured either white or black.
Penikisite ranges from blue to green with a vitreous luster and, when rubbed along a streak plate, can be pale green to white.
The surface across which the mineral is dragged is called a "streak plate," and is generally made of unglazed porcelain tile.
Sometimes a streak is more easily or accurately described by comparing it with the "streak" made by another streak plate.
In the absence of a streak plate, the unglazed underside of a porcelain bowl or vase or the back of a glazed tile will work.
In case of harder minerals, the color of the powder can be determined by filing or crushing with a hammer a small sample, which is then usually rubbed on a streak plate.
The streak plate method is a way to physically separate the microbial population, and is done by spreading the inoculate back and forth with an inoculating loop over the solid agar plate.
Because the trail left behind results from the mineral being crushed into powder, a streak can only be made of minerals softer than the streak plate, around 7 on the Mohs scale of mineral hardness.
Bacterial colonies from freshly streaked plates containing ampicillin at 100 g/ml and chloramphenicol at 25 g/ml were innoculated into YT-broth containing the above concentrations of antibiotics, and grown shaking at 37 C until the OD 450 was 0.5.