Sperm chromatin decondensation: 3 days abstinence prior to the test (unless otherwise instructed by the doctor).

It incorporates into sperm chromatin after fertilisation.

Disulfide bonds are also formed within and between protamines in the sperm chromatin of many mammalian species.

Another major function of nucleoplasmin is the decondensation of sperm chromatin at fertilization.

Special emphasis is placed on the discussion of the molecular mechanism involved in the sperm chromatin remodelling activity of this protein.

Acridine orange has been widely accepted and used in many different areas, such as epifluorescence microscopy, the assessment of sperm chromatin quality.

Reproductive: links to low birth weight and damage to sperm chromatin and DNA.

Key words: nucleoplasmin, review, histone chaperone, sperm chromatin remodeling, nucleosome assembly.

Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts.

Sperm Chromatin for the Researcher: A Practical Guide.

Key words: sperm chromatin, fertilization, Drosophila, Wolbachia, cytoplasmic incompatibility.

In Drosophila, the earliest effects caused by CI can already be observed during the sperm chromatin remodeling of the paternal chromosomes.

Sperm Chromatin Proteomics Identifies Evolutionarily Conserved Fertility Factors.

Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction.

Ashok is currently an editor of 27 highly acclaimed medical text books or manuals related to male infertility, ART, fertility preservation, sperm chromatin damage and antioxidants.

Biochemical studies using highly condensed Xenopus sperm chromatin and protein extracts prepared from multiple systems have lead to the identification of conserved proteins involved in chromosome decondensation.

Saccharomyces cerevisiae, chromosome decondensation, condensed Xenopus sperm chromatin, DNA, genetics, biochemistry, PKA.

The pentameric protein participates in various significant cellular activities like sperm chromatin remodeling, nucleosome assembly, genome stability, ribosome biogenesis, DNA duplication and transcriptional regulation.

We took a genetic approach to isolating chromosome decondensation mutants by incubating Xenopus sperm chromatin with whole-cell extracts prepared from the Hartwell library of random temperature sensitive (ts) yeast cells.

We show that decondensation of Xenopus sperm chromatin using wild type yeast extracts was rapid, ATP- and extract-dependent, and resistant to heat, N-ethylmaleimide, protease K, RNase A, and micrococcal nuclease.