Net fluid transport was calculated from registration periods lasting for at least 15 minutes and related to the serosal surface area measured after the experiment.

In the lidocaine experiments a 1% solution of the drug was applied to the serosal surface (0.5 mg/cm intestine).

In another type of experiment performed on days 6-9 after inoculation of the animals with the parasite, the serosal surface of the inflamed segment was exposed to lidocaine (1% solution).

The effect was described as "a grayish white, frost-like material of uric acid overlaying the serosal surfaces of the various organs, accompanied by sterile inflammation and necrosis in the affected and adjacent tissues" akin to avian visceral gout.

A plastic mould of 6 mm diameter was applied tightly to the serosal surface of the anterior wall of the stomach just proximal to the antral gland area and 70 l 100% acetic acid was poured through the mould on to the surface of the stomach for 20 seconds.