VAMP8 also participates the regulated exocytosis in pancreatic acinar cells.

Munc-18 proteins have been identified as essential components of the synaptic vesicle fusion protein complex and are crucial for the regulated exocytosis of neurons and neuroendocrine cells.

Regulated exocytosis, on the other hand, requires an external signal, a specific sorting signal on the vesicles, a clathrin coat, as well as an increase in intracellular calcium.

The diversity of sequences and structures flanking the calcium-coordinating amino acid residues renders the eight synaptotagmins bind to calcium at various affinities, covering the full range of calcium requirements for regulated exocytosis.