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No reducing agent or boiling are necessary since these would interfere with refolding of the enzyme.
Refolding of α-helical transmembrane proteins in vitro is technically difficult.
The letter was three weeks old, and already the cheap paper was splitting along the creases from constant refolding.
How are these proteins selected for this ATP-catalyzed refolding?
The simulation's results agreed with experimental observations and gave insights into the refolding of the dimer that were previously unobtainable.
The constant unfolding and refolding of the saris becomes a kaleidoscope of color and pattern.
This refolding renders the PrP isoform extremely resistant to proteolysis.
Carrera Pinto was waiting for the retiring division in order to join it and continuing refolding from the Andes.
The CJD prion is dangerous because it promotes refolding of native proteins into the diseased state.
A complex work of incising and refolding of the native atrial tissue is necessary to build the venous baffle.
Lewis Andersen, scrupulously honest, wore his habitual unrelieved black and was engaged in carefully refolding his napkin.
Palm and Rand McNally hasten to point out a final benefit: This atlas requires no refolding after use.
She unfolded the clothing she had brought, turning the blouse and skirt inside out before refolding them carefully and stacking them beside her daughter's body.
It has a positive effect on the refolding of denaturated lysozyme, with the refolding yield of about 90%.
In 2004, Folding@home was used to perform the first molecular dynamics study of the refolding of p53's protein dimer in an all-atom simulation of water.
Optical tweezers have been used to stretch single protein molecules from their C- and N-termini and unfold them and study the subsequent refolding.
However, DJ-1 is not just a chaperone for α-synuclein; it can also promote refolding of citrate synthase, glutathione transferase, and neurofilament light.
When a probe hybridizes to an open hairpin, complete refolding of the hairpin is stalled, and the position of the hybridized probe can be inferred.
The protein coded for by this gene is a small heat shock protein (sHSP) which protects bacteria from irreversible aggregation of proteins and aids in their refolding.
Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix.
States DJ, Dobson CM, Karplus M. A new two-disulphide intermediate in the refolding of reduced bovine pancreatic trypsin inhibitor.
These may include the folding of regions of protein structure that were previously unfolded or a re-arrangement in the folding or a refolding of the membrane-associated part of the proteins .
A stopped flow instrument coupled to either a circular dichroism spectrometer or a fluorescence spectrometer is often used in the field of protein folding, to observe rapid unfolding and/or refolding of proteins.
HSP 90 is required for ATP dependent refolding of denatured or unfolded proteins and for the conformational maturation of a subset of proteins involved in the response of cells to extracellular signals.
After applying a magnetic force, in the unzipped state of the hairpin, a small number of discriminating nucleotides can hybridize to the new individual complementary sequences on the hairpin which can transiently block the refolding of the hairpin.
Słownik biologiczny
