protein microarray

Figure 1 shows a forward (using antibody as a bait in here) and reverse phase protein microarray at the molecular level.

Protein microarray analysis can be used to detect subtle changes in protein activity in signaling pathways.

Nitrocellulose coated glass slides that are suitable for protein microarray applications are available from a number of commercial vendors.

To assess the applicability of this method to real-world samples, we examined protein microarray detection in various concentration ranges and background conditions.

This molecular tool may have applications for in vivo protein targeting, fluorescent microscopy, and irreversible attachment for a protein microarray.

Reverse phase protein microarray (RPPA) These arrays involve complex samples, such as tissue lysates.

Nevertheless, conventional white nitrocellulose films continue to be the dominant surface for many protein microarray applications because the claims above have not proved relevant to end user requirements.

This microarray format is very similar to DNA microarray, protein microarray and antibody microarray.

Many of the advantages of cell-free protein array technology address the limitations of cell-based expression system used in traditional methods of protein microarray production.

A protein microarray consists of a protein library immobilized on a substrate chip, usually glass, silicon, polystyrene, PVDF, or nitrocellulose.

Cell-free protein array technology attempts to simplify protein microarray construction by bypassing the need to express the proteins in bacteria cells and the subsequent need to purify them.

The high-throughput technology behind the protein microarray was relatively easy to develop since it is based on the technology developed for DNA microarrays, which have become the most widely used microarrays.

A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale.

The target proteins are synthesized in situ on the protein microarray, directly from the DNA template, thus skipping many of the steps in traditional protein microarray production and their accompanying technical limitations.

In 2006, another group refined and miniaturized this method by using multiple spotting technique to spot the DNA template and cell-free transcription and translation mixture on a high-density protein microarray with up to 13,000 spots (Figure 2).

Reverse phase protein microarray (RPMA) is a protein array designed as a micro- or nano-scaled dot-blot platform that allows measurement of protein expression levels in a large number of biological samples simultaneously in a quantitative manner when high-quality antibodies are available.