Gaps in the assembled sequence may be filled by primer walking.

The term "primer walking" is used where the main aim is to sequence the genome.

Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases.

Sequencing of 5' ends was performed on capillary sequencers (Molecular Dynamics); full-length sequencing was done on ABI377 sequencers using primer walking.

The M. pneumoniae genome sequence was published soon afterwards and was the first genome sequence determined by primer walking of a cosmid library instead of the whole-genome shotgun method.

We adopted a cDNA sequencing strategy that relies on an in vitro transposon insertion system based on the MuA transposase, combined with primer walking (see Materials and methods for details).