permeabilized

The NAD+ kinase activity of the permeabilized cells was stable and did not decrease after repeated use.

The ED-A antibody gave nearly identical results for permeabilized and nonpermeabilized cells.

This assumption was confirmed by experiments with permeabilized muscle preparations that were pre-contracted in a solution with pCa 5.5.

The participation of a protein kinase A dependent pathway during activation of permeabilized parietal cell is proposed.

In permeabilized cells in the presence of fructose-1,6-bisphosphate (20–2000 µM) aldolase shifts from association with actin fibres to intermediate filaments.

Channeling of carbamyl phosphate, the first intermediate in the pathway, has been demonstrated both in vitro and in permeabilized cells.

Oligonucleotide-directed RNAseH assays were performed with permeabilized viral cores under a wide variety of conditions.

The addition of trehalose to suspensions of permeabilized cells resulted in a sugar-dependent thermoprotection of the internal marker enzymes.

Characterization of nuclear import of potato spindle tuber viroid RNA in permeabilized protoplasts.

Binding of PGE2 to this receptor in permeabilized SL vesicles inhibits adenylyl cyclase activity.

However, DNA synthesis was ablated by micrococcal nuclease treatment of permeabilized cores, indicating that permeabilization was successful (data not shown).

The activity of the system was assayed in terms of levels of arginine deiminase, the signature enzyme of the system, in permeabilized cells.

When the extra-mitochondrial free calcium concentration was increased in permeabilized myocytes, the transmembrane potential was decreased proportionally, and this decrease was potentiated further by H2O2.

Treatment of permeabilized cells with phalloidin or viroisin induces, in the cortex of the cell, an intensive proliferation of filaments which have been identified as actin.

These results suggest that both in the permeabilized cells and in isolated membranes, the biosynthesis of phosphatidylcholine can be limited by both CDP-choline and diacylglycerol.

The CDP-choline: 1,2-diacylglycerol cholinephosphotransferase in permeabilized cells showed a Km of 88 μM for CDP-choline.

A similar approach was applied to histones modified by the endogenous poly(ADP-ribose) polymerase in permeabilized NS-1 mouse myeloma cells in culture.

Incubation of permeabilized cells with 150 μM acyl-CoA and 0.8 mM glycero-3-phosphate tripled cellular diacylglycerol levels, causing a doubling in the rate of phosphatidylcholine synthesis.

Permeabilized cell assay Permeabilized cell assays were performed in flow chambers as previously described [ 22 ] .

Furthermore, the DNA polymerase active site in permeabilized core particles is accessible to the exogenous RNAseH substrates (Fig.

For mock micrococcal nuclease treatment, the CaCl 2 micrococcal nuclease and EGTA were mixed prior to addition to the permeabilized cores.

If added prior to permeabilization but not during, exogenous actin was still able to incorporate into permeabilized cells and fluorescence incorporation was high, similar to control cells [Figure 1].

A similar change in sensitivity has been described for the InsP 3 -sensitive calcium channels in permeabilized liver cells, which spontaneously released their calcium when the stores were overloaded.

At the next level of organization, the action of InsP 3 has been studied on populations of receptors prepared either in membrane vesicles or in their normal location within permeabilized cells.

The permeabilized cells lost the bulk of the internal trehalose pool while most of the alkaline phosphatase, invertase, α-glucosidase, or neutral trehalase activities located inside the cells remained unaffected.