Western blot confirming expression of CFTR after transfection of HeLa cells using Lipofectin.

Cos-7 cells were transfected by calcium phosphate method, while the NIH3T3 cells were transfected using Lipofectin (Gibco, BRL).

The numbers of A549 cells transfected with 4003 significantly decreased with increasing concentration of 4003 compared to cells transfected either with a mismatch form of the 4003 or lipofectin control.

We have found DNA prepared this way to be immediately suitable for most laboratory procedures: both single and double-stranded sequencing reactions, restriction enzyme digestion, ligation, and for transfection of eukaryotic cells using lipofectin (data submitted but not shown).

ST cells grown to 50 % confluence in 35 mm dishes were transfected with 10 µg of plasmid DNA encoding CMV-driven minigenomes and 15 µl of Lipofectin reagent in Optimem medium (Gibco-BRL), according to the manufacturer's instructions.

HEK 293 cells were transfected with mouse μ opioid receptor cDNA in the pcDNA3 vector (a generous gift from Drs. Chris Evans and Duane Keith, UCLA) using the lipofectin reagent (Life Technologies, Rockville, MD).