However, there is a problem going in the other direction on the lagging strand.

The δ is involved in copying of the lagging strand.

However, significant challenges remain: the leading and lagging strands are anti-parallel.

Collectively, leading and lagging strand synthesis is referred to as being 'semidiscontinuous'.

The priming event on the lagging strand establishes a replication fork.

The main polymerase involved in lagging strand synthesis.

In contrast, lagging strand synthesis is accomplished in short Okazaki fragments.

When this is complete, a single nick on the leading strand and several nicks on the lagging strand can be found.

This makes the speed of lagging strand synthesis much lower than that of the leading strand.

This molecular process prevents the leading strand from overtaking the lagging strand.

DNA replication on the lagging strand is discontinuous.

This asymmetry is due the formation of the replication fork and its division into leading and lagging strands.

Electron microscopy studies indicate that nucleosome loading on the lagging strand occurs very close to the site of synthesis.

Thus, a section of the telomere is lost during each cycle of replication at the 5' end of the lagging strand.

This polymerase synthesises leading and lagging strand DNA in prokaryotes.

DNA ligase then joins the deoxyribonucleotides together, completing the synthesis of the lagging strand.

At this point, the lagging strand replicative polymerase associates with the clamp and primer in order to start polymerisation.

Any deviation from parity rule 2 will result in asymmetric base composition that discriminates the leading from the lagging strand.

On the leading strand this route is continuous, but on the lagging strand it is discontinuous.

Consider the following: the helicase continuously unwinds the parental duplex, but the lagging strand must be polymerised in the opposite direction.

This happens at all the sites of the lagging strand, but it does not happen at the end where the last RNA primer is attached.

Along the lagging strand's template, primase builds RNA primers in short bursts.

Occurs once at the origin on the leading strand and at the start of each Okazaki fragment on the lagging strand.

The leading strand receives one RNA primer while the lagging strand receives several.

Furthermore, the time used for replication between two strands varies and may lead to asymmetric mutational pressure between leading and lagging strand.