karyotyping

Learn about the purpose of Karyotyping, what to expect and how to prepare for this test.

The commonest reason for requesting karyotyping was the mother's age.

This method is also known as virtual karyotyping.

Array-based karyotyping can be done with several different platforms, both laboratory-developed and commercial.

Karyotyping is a test to identify chromosome abnormalities as the cause of malformation or disease.

Virtual karyotyping can also be done on fresh or formalin-fixed paraffin-embedded tumors.

Karyotyping is a blood test that looks for problems in the genetic material (chromosomes) in your cells.

Genome-wide assessment of copy number changes can be done by conventional cytogenetics or virtual karyotyping.

Karyotyping uses a system of studying chromosomes to identify genetic abnormalities and evolutionary changes in the past.

Karyotyping of a small number of these tumors revealed a common loss of chromosomes 9 and 11.

Virtual karyotyping can be performed on fresh or paraffin-embedded tumors to assess copy number at these loci.

Virtual karyotyping has the advantage of assessing the entire genome in one assay, as well as the 1p/19q loci.

Array-based karyotyping performs well on paraffin embedded tumors and is amenable to routine clinical use.

The status of the 1p/19q loci can be detected by FISH or virtual karyotyping.

HER2 amplification can be detected by virtual karyotyping of formalin-fixed paraffin embedded tumor.

Digital Karyotyping: Another method that provides a high resolution and high-throughput technology to quantify copy number of genes in samples.

Digital karyotyping is a technique used to quantify the DNA copy number on a genomic scale.

Alternatively, an indirect approach can be taken in which the entire genome can be assessed for copy number changes using virtual karyotyping.

Array-based karyotyping can be used to identify characteristic chromosomal aberrations in renal tumors with challenging morphology.

SNP-based arrays are preferred for virtual karyotyping of tumors and can be performed on fresh or paraffin-embedded tissues.

Karyotyping, and fluorescence in situ hybridization (FISH) are other techniques that can be used.

NB: Balanced translocations cannot be detected by array-based karyotyping (see Limitations below).

Johnson et al. "Preclinical validation of a microarray method for full molecular karyotyping of blastomeres in a 24-h protocol."

Microfluorimetry has uses for many different fields including cell biology, microbiology, immunology, cell cycle analysis and "flow karyotyping" of cells.

Virtual karyotyping has the added advantage of assessing copy number changes throughout the genome, in addition to detecting HER-2 amplification (but not overexpression).