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FISH can either be performed as a direct approach to metaphase chromosomes or interphase nuclei.
The multinucleated cells probably arise from failure of mitotic control, leading to variations in size and shape of interphase nuclei.
This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes.
It localises to euchromatin within interphase nuclei but is enriched within the centromeric heterochromatin of metaphase chromosomes.
Together with his brother Christoph Cremer he pioneered laser-UV-microirradiation experiments that indirectly implied a territorial organization of chromosomes in the interphase nucleus.
A biochemists' operational definition: Chromatin is the DNA/protein/RNA complex extracted from eukaryotic lysed interphase nuclei.
In transfected CHO cells, alphaB has also been shown to ectopically localize to interphase nuclei, suggesting a role for this protein in the nucleus [ 19 ] .
Upon incubation with serum containing anti-dsDNA antibodies and fluorescent labelled secondary antibodies, homogeneous staining of interphase nuclei and condensed chromosomal staining of mitotic cells can be seen.
Her major contributions include the discovery of complex repeated DNAs at centromeres and on chromosome arms (LINES) and their organization in interphase nuclei, in addition to elucidating mechanisms of infection in transmissible spongiform encephalopathies (TSEs).