The immunoreaction was revealed by a horseradish peroxidase-based chromogen.

The nucleus raphe pallidus has recently been shown to be involved in the activation of a fever as an immunoreaction.

Sites of immunoreaction were visualised by immersing sections in a solution of diaminobenzidine and hydrogen peroxide.

Alternatively, radioactive elements can be used as labels, and the immunoreaction can be visualized by autoradiography.

Use of one of these reagents after exposure to the necessary enzyme (e.g., horseradish peroxidase conjugated to an antibody reagent) produces a positive immunoreaction product.

The specificity of the immunoreaction was determined by preincubating the BMP-5 Ab with its specific blocking peptide (100 μg/ml) prior to reaction with the cultures.

For the PCNA immunoreaction, biopsy specimens were washed in PBS, flattened on a filter paper for better orientation after fixation, and fixed overnight in absolute methanol at 4 C.

Epithelial cells lining the airspaces adjacent to granulation tissue showed greater ET-1-IR (fig 2, panels A and B), whereas those adjacent to areas of dense fibrosis usually showed a weaker immunoreaction (fig 2, panel C).