By contrast, the time taken for labelled amylase to appear in the duodenal juice was longer in the patients (p<0.05).

There was no correlation between duodenal juice protein, enzyme turnover rates, and enzyme secretion.

There was no significant difference, however, in the time taken for labelled trypsin to appear in the duodenal juice of the post-acute pancreatitis patients.

The turnover rate of duodenal juice protein was 33% lower in the post-acute pancreatitis patients compared with controls (p<0.05)(Fig 4).

The stomach was washed out with water during a 10 minute rest period and the procedure was repeated with the patient's own bile stained duodenal juice.

Patients after acute pancreatitis had slower duodenal juice protein and amylase turnover rates but trypsin turnover was not different with controls.

He excluded patients with cystic fibrosis by performing duodenal drainage tests for the measurements of pancreatic enzyme concentrations in the duodenal juice.

Previous studies have attempted to evaluate the severity of duodenogastric reflux (DGR) and to determine whether specific constituents of duodenal juice are responsible for symptoms.

Despite this initial success the popularity of exfoliative cytology declined because of occasional reporting of false positive results, caused by degenerative cellular changes in the duodenal juice.

The relation between DGR and symptoms in patients with pain after gastric surgery was also investigated by a provocation test with the patient's own bile enriched duodenal juice.

Five ml samples of duodenal juice were mixed with an equal volume of 10% trichloroacetic acid (TCA) and trasylol (10% vol/vol) added to the remainder.

Amylase and trypsin were extracted from duodenal juice by affinity chromatography, permitting measurement of the rate of isotope incorporation into total protein, amylase, and trypsin.

To exclude such possible error a method was developed for the extraction of pure enzyme protein from aspirated duodenal juice, based on affinity chromatography for both amylase and trypsin.

Methods for the measurement of duodenal juice total protein specific radioactivities, pancreatic amylase and trypsin specific radioactivities, and plasma leucine specific radioactivities have been described.

About 70 ml of bile rich duodenal juice was collected by continuous low suction, and the AN20 tube was then repositioned into the stomach, about 48 cm from the insicors.

The role of the individual constituents of duodenal juice in the genesis of complications and of surgical procedures such as antireflux surgery and duodenal diversion in their prevention merits further investigation.

A four hour primed/continuous intravenous infusion of [C]L-leucine tracer was given with secretin (2 U/kg/h) and cholecystokinin (0.5 U/kg/h) and secreted duodenal juice aspirated.

In view of the fact that there is virtually no resistance to gastrooesophageal reflux in patients with Barrett's oesophagus, this increased duodenogastric reflux is likely to result in significant exposure of the oesophageal mucosa to duodenal juices.

Conversely, a negative provocation test suggests that the patient's pain is not due to the presence of irritant duodenal juice in the stomach, but could be associated with abnormal motility associated with DGR, or might be due to an unrelated cause.

The decreased total protein and amylase turnover rates are probably a result of a reduction in synthetic rates as the time taken for labelled total protein and amylase to appear in the duodenal juice was significantly longer in the the post-acute pancreatitis patients.

A balloon catheter was cannulated into the common bile duct through the cystic duct and a sample of 2-3 ml of hepatic bile was collected through the hole proximal to the inflated balloon to prevent contamination by pancreatic and duodenal juice.