DAPI (Sigma) was used for nuclear counterstaining.

Chromosome and probe denaturation, hybridization, detection, and counterstaining were all done according to standard protocols as recommended by the manufacturer.

Tissue sections were processed further as described previously [ 40 ] , including hematoxylin counterstaining of the nuclei.

In neurophysiological experiments which comprise calcium imaging methods, it can be used for a counterstaining of astrocytes to be able to analyze data from neurons separately.

Biotinylated anti-mouse immunoglobulins, streptavidin biotinylated peroxidase complex, DAB reaction, and the final counterstaining were conducted as described for the BrdU protocol.