cosmid

Those cells which did not take up the cosmid would be unable to grow.

Lane 1 corresponds to a cosmid from human chromosome 6 and serves as a control.

These particles- containing a linearized cosmid- are introduced into the host cell by transduction.

Phage library are also stored and screened easier than cosmid (colonies!)

Selection against wildtype cosmid DNA is simply done via size exclusion.

The cosmid, which is not packed correctly during mitosis, is occasionally present in only one daughter cell following cell division.

This causes linearization of the circular cosmid with two "cohesive" or "sticky ends" of 12bp.

Probes were hybridised to cosmid, YAC and P1 libraries.

It is a cosmid, and contains two functioning (wild-type) closely linked genes: a gene of interest and a mosaic marker.

The data from cosmid, P1 and YAC libraries were processed in parallel databases which were combined when required by the analysis programs.

There are advantages using a mapping strategy that integrates information from several clone libraries,(YAC, P1 and cosmid in the case of S.pombe ).

In addition,in situ hybridization of cosmid 2F10 to metaphase human chromosome spreads demonstrated a unique signal at the Xq21.3-Xq22 boundary (Fig.1 c).

Typically, Phrap had no problems differentiating between the different Alu copies in a cosmid, and to correctly assemble the cosmids (or, later, BACs).

DNA subclones and probes used in this work were isolated and subcloned from the appropriate cosmid or plasmid into Bluescript (Stratagene) following established techniques (34).

PCR, DNA sequence, and Southern blot data indicated that the recovered plasmid was the same as the cosmid integrated in the sur2 genome (data not shown).

Among 16 positive signals, we have detected an ERG cosmid confirmed by hybridisation with the cDNA probe (data not shown and RLDB).

A cosmid, first described by Collins and Hohn in 1978, is a type of hybrid plasmid (often used as a cloning vector) that contains a Lambda phage cos sequence.

The basic idea is that the cosmid probes that make up a contig should hit cosmid clones which are all hit by one or two YACs.

In situ hybridization was done as follows: biotin-labelled cosmid 2F10 and pSV2X5 were pre-annealed with Cot-1 DNA and then hybridized to metaphase spreads of cultured lymphocytes from a normal male.

The contigs of the cosmid and YAC libraries found by the two methods were essentially identical except for minor differences in order neighbouring probes having very similar or identical hybridisation patterns and which could be easily swapped.

The PCR product was used as a probe for colony blots to find cosmids in the E. coli (pYUB178::H37Rv) library carrying at least a portion of the cosmid integrated in the sur2 clone.

The cosQ site of next cosmid (as rolling circle replication often results in linear concatemers) is held by the terminase after the previous cosmid has been packaged, to prevent degradation by cellular DNases.

Results Cosmids carrying M. tuberculosis genes [ 20 ] were electroporated into M. smegmatis LR222 to create a library of M. smegmatis transformants, each of which carries a pYUB178::H37Rv cosmid integrated into its chromosome.