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He also worked in the field of amino acids and the Biuret reagent.
A further effort to prepare a colorless biuret reagent.
Not all biuret tests require the biuret reagent.
Copper(II) sulfate is also used in the Biuret reagent to test for proteins.
It is based on the biuret reagent, a blue solution that turns violet upon contact with proteins, or any substance with peptide bonds.
The traditional method for measuring total protein uses the biuret reagent, but other chemical methods such as Kjeldahl method, dye-binding and refractometry are now available.
It is a common precipitant in protein crystallography and is also an ingredient in the Biuret reagent which is used to measure protein concentration.
The Biuret reagent is made of potassium hydroxide (KOH) and hydrated copper(II) sulfate, together with potassium sodium tartrate.