ascending chromatography

The upper part of the Ascending chromatography can be folded over a rod and allowing the paper to become descending after crossing the rod.

Briefly, cells were scraped off the plates, centrifuged, the pellet extracted in chloroform:methanol:water and separated on high performance thin layer chromatography-silica plates (Mallinckrodt Baker Inc., Paris, KY) by ascending chromatography.