It can also be formed from pyruvate via anaplerotic reactions.

It is an important anaplerotic reaction that creates oxaloacetate from pyruvate.

It can arise from pyruvate via an anaplerotic reaction.

In this way, it enters the Krebs cycle, and is thus part of one of the anaplerotic reactions.

Glutamine can also be used to produce oxaloacetate during anaplerotic reactions in various cell types through "glutaminolysis" which is also seen in many c-Myc transformed cells.

Reactions that form intermediates of the TCA cycle in order to replenish them (especially during the scarcity of the intermediates) are called anaplerotic reactions.

Pyruvate is also converted to oxaloacetate by an anaplerotic reaction, which replenishes Krebs cycle intermediates; also, the oxaloacetate is used for gluconeogenesis.

Anaplerotic reactions can replenish the cycle at this juncture by synthesizing α-ketoglutarate from transamination of glutamate, or through action of glutamate dehydrogenase on glutamate.