This rescue process is believed to be dependent on the affinity of the antibody to the antigen.

These experiments are used to determine the specificity, amount, isotype and affinity of the antibodies produced.

To further improve binding affinity of the antibodies, the DNA fragments are denatured to produce single-stranded DNA.

An indirect approach is sometimes preferred when the concentration of the protein target is low or when the specific affinity of the antibody for the protein is weak.

A dose of 400 mg is used in digoxin poisoning, but a dose of 800 mg is recommended for oleandrin poisoning due to the lower binding affinity of the antibody to oleandrin.