AP site

Which means the sun shines out of her bits that you might stumble across on an AP site.

The phosphate group 3' to the AP site is stabilized through hydrogen bonding to Arg177.

If left unrepaired, AP sites can lead to mutation during semiconservative replication.

AP sites can be formed by spontaneous depurination, but also occur as intermediates in base excision repair.

APE1 contains several amino acid residues that enable it to react selectively with AP sites.

In E. coli, adenine is preferentially inserted across from AP sites, known as the "A rule".

I have submitted a pretty big petition for you to lose your job because your articles are so biased you don't deserve to be on an AP site.

They remove the damaged nitrogenous base while leaving the sugar-phosphate backbone intact, creating an apurinic/apyrimidinic site, commonly referred to as an AP site.

In alternative fashion, bifunctional glycosylase-lyases can cleave the AP site, leaving a 5' phosphate adjacent to a 3' α,β-unsaturated aldehyde.

Besides opening AP sites, they possess 3' phosphodiesterase activity and can remove a variety of 3' lesions including phosphates, phosphoglycolates, and aldehydes.

In this process, a DNA glycosylase recognizes a damaged base and cleaves the N-glycosidic bond to release the base, leaving an AP site.

BER is initiated by DNA glycosylases, which recognize and remove specific damaged or inappropriate bases, forming AP sites.

They flip the damaged base out of the double helix, as pictured, and cleave the N-glycosidic bond of the damaged base, leaving an AP site.

The AP endonucleases cleave an AP site to yield a 3' hydroxyl adjacent to a 5' deoxyribosephosphate (dRP).

This active site is bordered by Phe266, Trp280, and Leu282, which pack tightly with the hydrophobic side of the AP site, discriminating against sites that do have bases.

First, the Asp210 residue in the active site deprotonates a water molecule, which can then perform a nucleophilic attack on the phosphate group located 5' to the AP site.

The AP site can then be cleaved by an AP endonuclease, leaving 3' hydroxyl and 5' deoxyribosephosphate termini (see DNA structure).

Some lesions, such as oxidized or reduced AP sites, are resistant to pol β lyase activity and, therefore, must be processed by long-patch BER.

First, a DNA glycosylase cleaves the N-glycosidic bond, releasing the damaged base and creating an AP site- a site that lacks a purine or pyrimidine base.

Translesion synthesis is a DNA damage tolerance process that allows the DNA replication machinery to replicate past DNA lesions such as thymine dimers or AP sites.

AP endonuclease, specifically, catalyze the incision of DNA exclusively at AP sites, and therefore prepare DNA for subsequent excision, repair synthesis and DNA ligation.

In alkaline conditions, however, additional DNA structures are detected as DNA damage: AP sites (abasic sites missing either a pyrimidine or purine nucleotide) and sites where excision repair is taking place.

Its main role in the repair of damaged or mismatched nucleotides in DNA is to create a nick in the phosphodiester backbone of the AP site created when DNA glycosylase removes the damaged base.

In the next step, an AP endonuclease creates a nick at the 5' end of the AP site, generating a hanging deoxyribose phosphate (dRP) residue in place of the AP site.

In biochemistry and molecular genetics, an AP site (apurinic/apyrimidinic site), also known as an abasic site, is a location in DNA that has neither a purine nor a pyrimidine base, either spontaneously or due to DNA damage.