Petri dishes containing nutrient agar or other appropriate medium.
On nutrient agar, it forms unpigmented colonies.
A common identification assay involves culturing a sample of the organism deep within a block of nutrient agar.
Such a culture can be kept forever by successive transplantations onto fresh nutrient agar.
"Since 1925, the strain has remained on nutrient agar as a stock culture for teaching and research purposes," said Nature.
After the nutrient agar solidifies the plate is incubated.
Also, bacteria grown in nutrient agar grows on the surface, and is clearly visible as small colonies.
For definitive diagnosis, the pathogen should then be cultured on reduced nutrient agar.
It is composed of standard nutrient agar with the added ingredient of skim milk powder, which contains casein.
Cell pairs were removed, placed on nutrient agar and incubated in a warm (40-C) oven.