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He was also a pioneer in the study of microscopic specimens using vital stains.
Vital stains have been useful for diagnostic and surgical techniques in a variety of medical specialties.
The other measures are break up time, tear flow, hyperemia (redness, swelling), tear fluid cytology, and epithelial damage (vital stains) etc., which are human beings' physiological reactions.
Those stains excluded by the living cells but taken up by the already dead cells are called vital stains (e.g. trypan blue or propidium iodide for eukaryotic cells).
Very bulky or highly charged stains that don't cross live plasma membrane are used as vital stains and supravital stains are those that are either small or are pumped actively into live cells.
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