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This gene is a member of the DNA ligase family.
DNA ligase would most likely have to be incorporated and utilized in such a machine.
DNA Ligase then acts to join the two ends together.
The first DNA ligase was purified and characterized in 1967.
DNA ligase is then added to join the dye-labelled probe to the primer.
The strands are then rejoined with DNA ligase in a quick and efficient process.
The insert and the destination vector are then mixed together with DNA ligase.
The fragments are then inserted into the vector using the enzyme, DNA ligase.
Most solutions involve an enzyme called DNA ligase.
DNA ligase joins the fragments together.
The single-stranded nicks are ligated by host DNA ligase.
DNA ligase then joins the deoxyribonucleotides together, completing the synthesis of the lagging strand.
Once paired, the phosphodiester bonds of the fragments can be joined by DNA ligase.
DNA ligase can repair a break in a DNA strand.
They anneal to the 3C fragments and get ligated together with a DNA ligase.
Experimental genomic evolution: extensive compensation for loss of DNA ligase activity in a virus.
Once inside the host, the cosmids circularize with the aid of the host's DNA ligase and then function as plasmids.
A mismatch at the 3' end of the discriminating primer prevents the DNA ligase from joining the two fragments together.
Restriction endonucleases and DNA ligase are then used to clone the sequences into bacterial plasmids.
Eukaryotic DNA ligase 1 catalyzes a reaction that is chemically universal to all ligases.
Finally, the completion of the dsDNA is carried out by DNA ligase.
Sequencing by ligation relies upon the sensitivity of DNA ligase for base-pairing mismatches.
DNA ligase is an enzyme that joins together ends of DNA molecules.
DNA Ligase then joins the various fragments together into a continuous strand of DNA.
He then did his postdoctoral training with Arthur Kornberg and purified the T4-phage DNA ligase.