Słownik angielskiego

Analysis of gene expression Total RNA isolated from the cell lines was reverse transcribed using random primers and the Pro-STAR first strand RT-PCR kit (Stratagene, La Jolla, CA).

The RNA is reverse transcribed with either polyT primers which amplify only mRNA or random primers which amplify all RNA which is mostly rRNA, miRNA microarray ligate an oligonucleotide to the purified small RNA (isolated with a fractionator) and then RT and amplified.

An RNA oligonucleotide was then ligated to the full-length, decapped mRNAs and reverse transcription was performed using random primers.

RNA samples (5 μg) were reverse transcribed using random primers at annealing temperatures of 65 C (You-Prime-the-First-Strand kit, Amersham, Piscataway, NJ).

RNA was first mixed with either 8 μg of random primer for aRNA or 5 μg of oligo(dT) primer for poly(A) +RNA in 16 μl of RNase-free water.

It is used to analyse the genetic diversity of an individual by using random primers.

Northern blot analysis was carried out using double-stranded DNA probes labeled to a specific activity of 10 8- 10 9cpm/μg with α-[ 32P] CTP using T7 DNA polymerase and random primers.

It is important to note that our protocol for generating first-strand cDNA target involves the use of random primers.

Total RNA isolated from treated and untreated cell lines, and the total RNA and poly A +RNA from normal cervix obtained from Ambion (Austin, TX) was reverse transcribed using random primers and the Pro-STAR first strand RT-PCR kit (Stratagene, La Jolla, CA).

Direct labeling Unamplified DNA samples for labeling were generated from 1 μg restricted DNA using Klenow fragment and random primers, and incorporating amino-allyl dUTP, as described at http://www.microarrays.org.