Also the retinal structural reliability improved greatly after the treatment, characterized by an increase in the outer nuclear layer thickness.

Microdissected outer nuclear layer tissue was obtained by flat-mounting freshly dissected retinas flattened by radial cuts onto nitrocellulose filters.

Materials and methods Isolation of mouse brain and retinal tissue C57/B6 mice were used to obtain tissue from hypothalamus, adult retina and outer nuclear layer.

In addition, the outer nuclear layer (ONL) in nocturnal mammals is thick due to the millions of rods present to process the lower light intensities of a few photons.

It consists of a dense network of synapses between dendrites of horizontal cells from the inner nuclear layer, and photoreceptor cell inner segments from the outer nuclear layer.

The histological examination of the eyes of one such patient showed that the outer nuclear layer of cones and rods had disappeared completely, whereas the RPE showed pronounced pigment changes.

The outer nuclear layer (or layer of outer granules or external nuclear layer), is one of the layers of the vertebrate retina, the light-detecting portion of the eye.

Orthogonal cryostat sections of 20 μm were cut onto Superfrost slides (Fisher), and the edge of the section containing the outer nuclear layer was hand dissected using a microscalpel and immediately frozen.

All photoreceptors in humans are found either in the outer nuclear layer in the retina at the back of each eye, while the bipolar and ganglion cells that transmit information from photoreceptors to the brain are in front of them.

The procedure involved injecting rod precursors which formed an 'anatomically distinct and appropriately polarized outer nuclear layer' - two weeks later a retina had formed with restored connections and sight, proving that it was possible to reconstruct the entire light-sensitive layer.

As a baseline gauge of variability in tag sampling, we compared the collection of tags comprising the first 26,000 tags obtained from the microdissected outer nuclear layer library to the collection of tags comprising the second 26,000 tags obtained from this library.

Like the inner nuclear layer, the outer nuclear layer contains several strata of oval nuclear bodies; they are of two kinds, viz.: rod and cone granules, so named on account of their being respectively connected with the rods and cones of the next layer.

We typically saw higher expression of photoreceptor-specific genes in microSAGE libraries made from microdissected outer nuclear layer (97% rods versus 70% rods for whole retina) and, in many cases, higher expression in P10.5 crx +/+samples relative to P10.5 crx -/-samples.